|
KMID : 0861020130280020055
|
|
Korea Journal of Herbology
2013 Volume.28 No. 2 p.55 ~ p.60
|
|
|
Inhibitory Effects of Chestnut Inner Shell Cytokine Production from Human Mast Cell Line
|
|
전동하:Jun Dong-Ha
장영아:Jang Young-Ah/김희영:Kim Hui-Yeong/김수진:Kim Su-Jin/이진태:Lee Jin-Tae/김진철:Kim Jin-Chul/김세현:Kim Sea-Hyun/권대준:Kwoen Dae-Jun/한상익:Han Sang-Ik
|
|
|
Abstract
|
|
|
Objectives : Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by eczematous
inflammtion of the skin. The chestnut inner shell extracts (CI) has been used as a cosmetic material for a long
time in Korea. However, the precise anti-allergy effects of CI have yet to be clearly elucidated. In this study,
we attempted to evaluate the effect of CI on mast cell-mediated allergy inflammation.
Methods : To find the anti-allergy and inflammatory effect of CI, we investigated the inhibitory effect of CI on
the production of inflammatory mediators using by enzyme-linked immunosorbent assay in phorbol 12-myristate
13-acetate (PMA) plus calcium ionophore (A23187) stimulated-human mast cell (HMC-1).
Results : In this study, we found that CI did not show cytotoxic effect at up to 10 ug/ml on HMC-1. CI inhibited
the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in stimulated HMC-1. Maximal
rate of TNF-α, IL-6 and IL-8 inhibition by CI (10 ug/ml) were about 47.6%, 44.1% and 22.5% respectively. In
addition, we showed that Fr.3 isolated from n-Butyl alcohol layer of CI attenuated the production of TNF-α,
IL-6 and IL-8 in HMC-1.
Conclusion : Taken together, the findings of this study provide us with a novel insight action of CI as a potential
molecule for use in the treatment of allergic inflammation diseases.
|
|
|
KEYWORD
|
|
|
Chestnut Inner Shell, HMC-1, Inflammatory cytokine, Allergy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|